Porphyromonas pasteri and Prevotella nanceiensis in the sputum microbiota are associated with increased decline in lung function in individuals with cystic fibrosis

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dc.contributor.affiliationUniversity of Nottingham; Nottingham MRC Molecular Pathology Node; King's College London; University Hospitals Birmingham NHS Foundation Trusten_US
dc.contributor.authorWebb, Karmel
dc.contributor.authorZain, Nur Masirah M
dc.contributor.authorStewart, Iain
dc.contributor.authorFogarty, Andrew
dc.contributor.authorNash, Edward F
dc.contributor.authorWhitehouse, Joanna L
dc.contributor.authorSmyth, Alan R
dc.contributor.authorLilley, Andrew K
dc.contributor.authorKnox, Alan
dc.contributor.authorWilliams, Paul
dc.contributor.authorCámara, Miguel
dc.contributor.authorBruce, Kenneth
dc.contributor.authorBarr, Helen L
dc.contributor.departmentRespiratory Medicineen_US
dc.contributor.roleMedical and Dentalen_US
dc.contributor.trustauthorWhitehouse, Joanna
dc.date.accessioned2024-10-22T11:40:52Z
dc.date.available2024-10-22T11:40:52Z
dc.date.issued2022-02-03
dc.description.abstractAlthough anaerobic bacteria exist in abundance in cystic fibrosis (CF) airways, their role in disease progression is poorly understood. We hypothesized that the presence and relative abundance of the most prevalent, live, anaerobic bacteria in sputum of adults with CF were associated with adverse clinical outcomes. This is the first study to prospectively investigate viable anaerobic bacteria present in the sputum microbiota and their relationship with long-term outcomes in adults with CF. We performed 16S rRNA analysis using a viability quantitative PCR technique on sputum samples obtained from a prospective cohort of 70 adults with CF and collected clinical data over an 8 year follow-up period. We examined the associations of the ten most abundant obligate anaerobic bacteria present in the sputum with annual rate of FEV1 change. The presence of Porphyromonas pasteri and Prevotella nanceiensis were associated with a greater annual rate of FEV1 change; -52.3 ml yr-1 (95 % CI-87.7;-16.9), -67.9 ml yr-1 (95 % CI-115.6;-20.1), respectively. Similarly, the relative abundance of these live organisms were associated with a greater annual rate of FEV1 decline of -3.7 ml yr-1 (95 % CI: -6.1 to -1.3, P=0.003) and -5.3 ml yr-1 (95 % CI: -8.7 to -1.9, P=0.002) for each log2 increment of abundance, respectively. The presence and relative abundance of certain anaerobes in the sputum of adults with CF are associated with a greater rate of long-term lung function decline. The pathogenicity of anaerobic bacteria in the CF airways should be confirmed with further longitudinal prospective studies with a larger cohort of participants.en_US
dc.identifier.citationWebb K, Zain NMM, Stewart I, Fogarty A, Nash EF, Whitehouse JL, Smyth AR, Lilley AK, Knox A, Williams P, Cámara M, Bruce K, Barr HL. Porphyromonas pasteri and Prevotella nanceiensis in the sputum microbiota are associated with increased decline in lung function in individuals with cystic fibrosis. J Med Microbiol. 2022 Feb;71(2):001481. doi: 10.1099/jmm.0.001481.en_US
dc.identifier.doi10.1099/jmm.0.001481
dc.identifier.eissn1473-5644
dc.identifier.issn0022-2615
dc.identifier.pmid35113780
dc.identifier.urihttp://hdl.handle.net/20.500.14200/6184
dc.language.isoenen_US
dc.publisherMicrobiology Societyen_US
dc.relation.urlhttps://www.microbiologyresearch.org/content/journal/jmmen_US
dc.source.countryUnited Kingdom
dc.source.countryUnited Kingdom
dc.source.countryEngland
dc.source.issue2
dc.source.journaltitleJournal of Medical Microbiologyen_US
dc.source.volume71
dc.subjectMicrobiology. Immunologyen_US
dc.subjectRespiratory medicineen_US
dc.titlePorphyromonas pasteri and Prevotella nanceiensis in the sputum microbiota are associated with increased decline in lung function in individuals with cystic fibrosisen_US
dc.typeArticleen_US
dspace.entity.typePublication
oa.grant.openaccessnaen_US
rioxxterms.versionNAen_US
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